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Sometimes this issue is fixed simply by optimizing buffers, for instance by increasing salt or detergent concentration, so that the interacting protein no longer binds to the GST only control, but still binds to your GST-fusion protein of interest
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A From the total glutathione (GSH + GSSG) and GSSG concentrations obtained from the calibration curve, the GSH concentration is calculated using the following formula
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Induction of autophagy via the ROS-dependent AMPK-mTOR pathway protects copper-induced spermatogenesis disorder
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